However, there appear to be no citrus packing machine CTV-free areas among
the Hawaiian Islands where quarantine procedures for new plantings might be
implemented. Removing infected plants to establish CTV-free areas and replacing
them with virus-free planting material would also be ineffective, as
viruliferous brown citrus aphids would soon re-infest the virus-free plantings.
Mild CTV strains that confer cross
protection against severe CTV strains are well established in some
citrus-production areas in Hawai‘i. citrus packing machine Many productive trees
in Hawai‘i are infected with these cross-protecting strains.
Resistant or tolerant citrus varieties such as pummelo (C. maxima Merr.)
and some mandarins (C. reticulata L.), popular in local markets, may be
grown productively.
Quarantine, certification, and suppression/ eradication programs
Quarantine programs test incoming citrus
planting stocks for CTV and other citrus pathogens that may harm a region’s
citrus industry. citrus packing machine
Citrus trees are usually clonally propagated by grafting, and CTV is graft-transmissible.
Thus, an important aspect of CTV management is maintaining CTV-free bud stocks.
Buds from a single tree can be used to produce hundreds of new plants that may
be distributed over great distances. Many CTV epidemics were started or citrus packing machine enhanced by the
distribution of infected budwood (3). Budwood certification programs are
designed to keep such budwood free of CTV and other graft-transmissible
pathogens. In such programs, citrus packing machine primary budwood source
trees are regularly monitored for infections and may be further protected by
insect-proof screening, or moved to locations far removed from CTV-infected
trees (29). In order to reduce the number citrus packing machine of
CTV-infected trees in a region, surveys are routinely conducted to detect
infected trees and have them removed. Outside of Hawai‘i, such suppression
programs are generally undertaken only if the CTV incidence is However, determining the incidence of CTV in a region is difficult
where citrus packing machine disease
incidence is low, as a large number of samples must be processed to accurately
estimate disease incidence. Several survey techniques have been designed to
maximize the area and number of trees sampled, while minimizing citrus packing machine the
number of samples processed, often by combining samples from adjacent trees
(41). If any of these combined samples are positive, a more intensive survey is
conducted within the affected area to determine the extent of the infection.
Although these are often called “eradication” programs, usually citrus packing machine not
all infected trees are removed, given the time lag between initial infection
and when the virus is detectable by laboratory assays. If successfully
implemented, however, a CTV-suppression program can be cost effective and may
keep CTV at acceptable levels (29).
The
control methods described above can also be applied to regions where so-called
mild strains of CTV are common and where the goal is to control severe strains.
Strain-selective control approaches, however, citrus packing machine
require a method to discriminate between the more desirable strains and those
severe strains to be eradicated. In Florida, which has populations of both mild
and disease-inducing strains, control is focused mainly on managing the more
severe, stem-pitting strains of CTV. An important component citrus packing machine of this management
strategy is the use of the antibody MCA-13, which can discriminate between mild
and severe Florida strains (62) to screen trees. Trees testing positive for
MCA-13 are identified for removal by the testing process. This reduces the effort
and expense of removing a large number of trees, and growers do not lose
productive trees whose health is strengthened by the protective effects of
infection by milder strains of CTV. Additionally, budwood harboring CTV
infections that citrus packing machine are not reactive to
MCA-13 can be propagated and distributed on a limited basis (29). A similar
strain-selective control strategy was employed in California when a
stem-pitting strain was introduced at a single
location. Although no antibodies were available that could discriminate between
the introduced strain and endemic mild strains, indicator plants were used to
identify infected trees. These trees citrus packing machine were removed and the introduced strain of CTV was eradicated
(29).



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